Enzyme immunoassay (ELISA) for the measurement of levels of human growth hormone in serum and plasma. Human growth hormone measurements are used in the diagnosis and treatment of disorders involving the anterior lobe of the pituitary gland. For research use only, not for use in diagnostic procedures.<br><br>
The endocrine system of human Growth Hormone (hGH), also named Somatropin, is characterized by an extreme complexity. hGH is the product of the GH-1 gene located on chromosome 17 and expressed in pituitary cells. 80% of the hGH is a non-glycolsylated 22 kDa protein consisting of 191 amino acids. About 20% is a variant form of 20 kDa resulting from alternative splicing. Additionally, several smaller variants can be found in circulation as well as translational modificated proteins and different degrees of protein aggregation. Bioactivity of Growth Hormone is regulated by a specific binding protein (GHBP) formed by the extra cellular part of the cellular transmembran GH-receptor. These modifications allow a tight control of the half-life period hGH and of its bioactivity.<br><br>
Not only synthesis and posttranslational modification but also secretion of hGH is tightly regulated. Spontaneous pulsatile secretion takes place with a single pulse every three hours and a maximal secretion during night’s sleep. Several different stimuli as physiologic stress or hypoglycaemia result in additional hGH secretion, induced by the hypothalamic hormones Somatostatin and GH-Releasing Hormone (GHRH). The amount of secreted GH is influenced by multitude of factors (see References 2-5).
- Assay Description:
- 2 hour incubation (RT) + 30 min. (RT) + 30 min. (RT) + 15 min. (RT) = 3 hours, 15 min. total incubation time
- Catalog number:
- E022
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 control, lyophilized
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum and plasma
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 5 standards, lyophilized
- Standard range:
- 0.05 - 1.0 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.0115 ng/mL
- Species:
- Human
