Hepatic Triglyceride Lipase (HTGL)

Solid phase sandwich ELISA for the determination of HTGL in human serum, EDTA plasma and post-heparin plasma. For research use only, not for use in diagnostic procedures.<br><br>

HTGL (hepatic triacylglycerol lipase / hepatic triglyceride lipase) is a secreted glycoprotein and known as lipolytic enzyme and is also called as hepatic lipase (HL). HTGL has an important role in lipoprotein metabolism as a lipolytic enzyme that hydrolyzes triglycerides (TG) and phospholipids in chylomicron remnants, intermediate-density lipoproteins (IDL) and high-density lipoproteins (HDL). It has been reported that hypercholesterolemia or triglyceridemia is observed and β-very-low-density lipoproteins (VLDL), chylomicron remnants, IDL, TG-rich low-density lipoproteins (LDL) and HDL are accumulated in patients with deficiency of HTGL. HTGL is synthesized by hepatocytes and bound to heparin sulfate proteoglycans at the surface of liver sinusoidal capillaries.<br><br>

In general, it is essential for use heparin plasma that is collected after intravenous injection of heparin (post-heparin plasma) for measurement of HTGL in blood sample as same as lipoprotein lipase (LPL) as HTGL that bonds and adheres to surface of endothelial cells is removed from capillary vascular bed  and released in blood stream soon after the heparin injection. This ELISA kit can detect HTGL in serum and plasma regardless with or without administration of heparin injection that is conventionally required for the measurement.