Enzyme Immunoassay (ELISA) for the determination of Histamine in different animal species and biological fluids.<br><br>
During the sample preparation Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450nm.<br><br>
Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 45 min. incubation (RT) + 15 min. (RT) + 3 hours (RT) + 30 min. (RT) + 20 min. (RT) = 4 hours, 50 min. total incubation time
- Catalog number:
- IB89142
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Please Inquire
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Different animal species and sample types - please see protocol
- Sample volume:
- 25 µL of properly diluted unknown / determination
- standards:
- 6 calibrators, ready to use
- Standard range:
- 0 / 0.5 - 50 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.2 ng/mL
