Solid phase sandwich ELISA for the determination of human Intact Angiotensinogen ELISA in serum, EDTA-plasma, and urine. For research use only, not for use in diagnostic procedures.<br><br>
Angiotensinogen is the precursor of angiotensin and is cleaved into angiotensin I and II in the renin-angiotensin system, and it has long been reported to play an important role in controlling blood pressure. In recent years interest related to the role of the renin-angiotensin system in arterial pressure control and the pathophysiology of hypertension has been shifting to its local role in various tissues. Among the studies urinary excretion of angiotensinogen in a rat model of angiotensin II (AII)-dependent hypertension has been reported to be a marker of the activity of the local intrarenal renin-angiotensin system. Intrarenal AII increases to an extent in AII-dependent hypertension that cannot be explained by the plasma AII equilibration alone, and two mechanisms, an increase in intracellular uptake of AII and an increase in intrarenal expression of angiotensinogen, have been proposed to explain it. <br><br>
This kit can quantitatively measure Human Intact (Full-Length) Angiotensinogen in Serum, EDTA-plasma and Urine. <br><br>
These samples should be diluted with appropriate dilution ratio prior conducting the assay. The dilution ratio should be determined by each laboratory.
- Assay Description:
- 1 hour incubation (37°C) + 30 min (37°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27742
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA plasma, and urine
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0.28 - 18 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.093 ng/mL
- Species:
- Human
- Additional info: