Enzyme immunoassay for detection of CTSD concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only. Not for use in diagnostic procedures.
Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins . It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 10-20 min. (37°C) = 3 hour, 20min. total incubation time
- Catalog number:
- IB08047
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Serum, plasma, tissue homogenates and other biological fluids
- Sample volume:
- 100 μL of properly diluted sample
- standards:
- 8 standards, from 1 vial of lyophilized stock
- Standard range:
- 0 / 46.875 - 3000 pg/ml
- sensitivity:
- 28.125 pg/ml
- storage:
- 2-8 °C
- Species:
- Human
