Immunoradiometric assay (RIA) for the determination of human growth hormone (hGH) in serum. FDA registered 510(k) Exempt. For In-Vitro Diagnostic Use
hGH is a polypeptide hormone (molecular weight 21,500 Da) produced by the acidophil cells of the anterior pituitary under the control of two main substances from the median eminence : Growth-hormone Releasing Factor (GRF) and an inhibitory agent, somatostatin. Dopaminergic, adrenergic and serotoninergic neuroendocrine pathways also play an important role in the control of hGH secretion. Excitatory stimuli of hGH secretion include hypoglycemia, exercise, fasting, meals with a high protein content, deep sleep, stress, glucagon, L Dopa, amino acids, etc. Inhibitory stimuli include glucose, cortisol, hGH and free fatty acids. Because of its short plasma half life (±25 minutes) and of the frequent excitatory or inhibitory stimuli, hGH displays frequent and large variations of concentration in serum.
One of the main physiological functions of hGH is to act on the liver and other tissues to produce Somatomedins, which in turn induce growth by direct action on target tissues. In contrast to hGH, the concentration of somatomedin in serum is kept stable by virtue of being largely bound to circulating plasma proteins.
- Assay Description:
- 2 hours total incubation time
- Catalog number:
- KIP1081
- configuration:
- Reagents sufficient for 96 determinations
- controls:
- 2 controls, lyophilized
- design:
- Radioimmunoassay (RIA) technique
- FDA Status:
- Registered IVD
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Human growth hormone (hGH)
- Protocol:
- Sample types:
- Serum
- Sample volume:
- 50 μL / determination
- standards:
- 7 calibrators, lyophilized
- Standard range:
- 0 / 1 - 120 μIU/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.04 μIU/mL
- Species:
- Human
- radioactivity:
- 9.5 µCi (350 kBq)
