Enzyme immunoassay for determination of Human IL-2 concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only, not for use in diagnostic procedures.
Uniprot ID： P60568
Interleukin 2 (IL 2) is a O glycosylated four alpha helix bundle cytokine that has potent stimulatory activity for antigen activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils. It shows strain specific heterogeneity in an N terminal region that contains a poly glutamine stretch. Mouse and human IL 2 exhibit cross species activity. The receptor for IL 2 consists of three subunits that are present on the cell surface in varying preformed complexes. The 75 kDa IL 2 R beta, which is also a component of the IL 15 receptor, binds IL 2 with intermediate affinity. It drives resting T cells to proliferate and induces IL 2 and IL 2 R alpha synthesis.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 10-20 min. (37°C) = 3 hours, 20 min. total incubation time
- Catalog number:
- IB08130
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immunosorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Interleukin-2 (IL2), T-cell growth factor
- Sample types:
- Serum, plasma, cell culture supernatant, and other biological samples
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 15.625 - 1000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 9.375 pg/mL
- Species:
- Human
