Solid phase sandwich ELISA for the determination of human LRG in serum, EDTA plasma. For research use only, not for use in diagnostic procedures.
Leucine rich alpha2-glycoprotein (LRG) is one of leucine-rich repeat family proteins. LRG has been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. Recently, it was reported that serum levels of LRG were significantly elevated in Rheumatoid arthritis (RA) patients compared to healthy controls and decreased after anti-TNF therapy. Another report indicates serum LRG levels are elevated in other inflammatory diseases compared to healthy controls. LGR is expected as an inflammatory marker in various inflammatory diseases.
The ELISA kit correlates significantly the current product (P/N 27769) and have benefits of shorter assay time and higher sensitivity than the current product. The current product will be discontinued when the stocks lasts.
- Assay Description:
- 60 min. (37°C) + 30 min. (2-8°C) + 30 minutes (RT) = 2 hour total incubation time
- Catalog number:
- 27798
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- MSDS:
- Available upon request
- Sample types:
- Serum and EDTA plasma
- Sample volume:
- 100 μL of properly diluted unknown / determination
- EIA Buffer:
- Additional EIA buffer may be required for samples that require a higher dilution. The buffer can be ordered under product code 27798D100. This is Human LRG (Mono) EIA buffer with 100mL volume and should only be used with product 27798. Please inquire
- Recommended Dilution for Serum:
- The suitable dilution factor may vary by the condition, but generally a dilution around 5,000X is used for measurement.
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 1 - 64 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.06 ng/mL
- Species:
- Human
- Expression of Standard:
- Recombinant protein expression using CHO cells.
- Epitope of Plate-Coated Antibody:
- Antibody is from recombinant protein antigen. No epitope mapping has been conducted.
- Epitope of Labeled Antibody:
- Antibody is from recombinant protein. No epitope mapping has been conducted.
- Additional info:
