Solid phase sandwich ELISA for the detection of Human Lipoprotein Lipase (LPL) in serum, EDTA-plasma, heparin plasma, and post-heparin EDTA-plasma. <br><br>
LPL is an enzyme which catabolizes Triglyceride (TG) in triglyceride-rich lipoproteins such as dietary-derived Chylomicron and VLDL synthesized in the liver and it has an important role for TG metabolism with as the essential factor ApoC-II. <br><br>
LPL is bound by GPIHBP1 or heparan sulfate proteoglycan on the surface of microvascular endothelium of adipose tissue rather than the liver or muscles.<br><br>
This kit can quantitatively measure LPL in human serum, EDTA plasma, heparin plasma and post heparin EDTA plasma. <br><br>
- Assay Description:
- 60 min. incubation (37°C) + 30 min. (4°C) + 30 min. (RT) = 2 hour total incubation time
- design:
- Enzyme immunoassay (ELISA) technique
- Catalog number:
- 27268
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Serum, EDTA-plasma, Heparin plasma, Post-heparin EDTA-plasma
- standards:
- 7 standards, prepared from stock material
- Standard range:
- 0 / 0.02 - 1.5 ng/mL
- storage:
- 2 - 8°C
- sensitivity:
- 0.004 ng/mL
- Species:
- Human