Enzyme immunoassay for determination of MMP-1 concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only, not for use in diagnostic procedures.
Uniprot ID： P03956
The matrix metalloproteinase (MMP) family of proteases is a group of zinc-dependent enzymes that target extracellular proteins, including growth factors, cell surface receptors, adhesion molecules, matrix structural proteins, and other proteases . Within this family, MMP-1, MMP-8, and MMP-13 have been characterized as a collagenase subfamily of MMPs targeting fibrillar collagen (collagen type I, II, and III) for degradation. In addition to collagen, MMP-1 also has activity toward a broad array of other ECM proteins, such as fibronectin, gelatin, aggrecan (etc.), as well as growth factors, chemokines, and cytokines . MMP-1 is widely involved in tissue remodeling during wound healing, tumor growth, invasion and metastasis, and arthritis .
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 10-20 min. (37°C) = 3 hours, 20 min. total incubation time
- Catalog number:
- IB08097
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immunesorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Other names:
- Matrix metalloproteinase-1 (MMP-1), Interstitial collagenase, Fibroblast collagenase
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Human serum, plasma, cell culture supernatant and other biological samples
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 78.125 - 5000 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 46.875 pg/mL
- Species:
- Human
