α-Klotho (Alpha Klotho) was identified as an extremely down-regulated gene in the genetically-modified mouse of which phenotype is very similar to various symptoms of human aging. The sequences of Alpha Klotho genes have been identified in various species including humans based on the one of mouse. Alpha Klotho protein is a 130 kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that Alpha Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. It is considered that in Alpha Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of Alpha Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of Alpha Klotho protein is released free into blood by shedding.
There is also a secreted Alpha-Klotho that has only first repeat due to gene splicing.
However, there are many unclear points regarding the function of secreted Alpha-Klotho protein and its concentration fluctuation, so it was required to develop the measurement system of Alpha Klotho. This kit specifically measures human secreted isoforms (secreted Alpha-Klotho) and does not react with soluble Alpha Klotho proteins derived from membrane Alpha Klotho.
- Assay Description:
- Overnight (ON) incubation (2-8℃) + 60 min. (2-8℃) + 30 min. (RT) = ON + 1 hour 30 min. total incubation time
- Catalog number:
- 27901
- configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- MSDS:
- Please Inquire
- Notes:
- The protocol for the secreted Alpha Klotho product (see above) is intended to serve as an example only. Please refer to the instructions for use provided with the assay kit for precise details.
- Sample types:
- Serum, EDTA-plasma, and cerebrospinal fluid (CSF)
- Sample volume:
- 100 µL of properly diluted unknown / determination
- Standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 70.31 - 4500 pg/mL
- storage:
- 2 - 8 °C
- Sensitivity:
- 9.87 pg/mL
- Species:
- Human
- Pamphlets:
