Enzyme immunoassay for determination of Human TNF-α concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only, not for use in diagnostic procedures.
Uniprot ID： P01375
TNFα also known as Tumor Necrosis Factor alpha. It is mainly secreted by various immune cells, such as monocytes, macrophages, and T cells, and plays an important role as a regulatory factor in inflammation and immune responses. When the body is subjected to trauma, infection, tumors, and other damage, TNFα can stimulate inflammation and activation of immune cells, and participate in resisting external invasion. However, excessive or abnormal release of TNFα can also cause a series of diseases, such as rheumatoid arthritis, inflammatory bowel disease, autoimmune diseases, and others.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 10-20 min. (37°C) = 3 hours, 20 min. total incubation time
- Catalog number:
- IB08064
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immunosorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- TNF-α, Tumor Necrosis Factor alpha
- Sample types:
- Serum, plasma, cell culture supernatant, and other biological samples
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 15.625 - 1000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 9.375 pg/mL
- Species:
- Human
