Solid phase sandwich ELISA for the determination of human Uromodulin in serum and EDTA plasma. For research use only, not for use in diagnostic procedures.<br><br>
Uromodulin is a highly organ-specific protein that is produced only in the renal tubules of the kidney. Almost all of uromodulin is excreted in the urine, but a small amount is also present in the blood. Uromodulin excreted in urine may be excreted as a single protein, or it may aggregate and be excreted as hyaline casts, depending on the nature of the urine and the state of the disease. On the other hand, it is reported that uromodulin in serum is more suitable for quantitative measurement because it is often excreted as a single substance.
- Assay Description:
- 60 min. incubation (37°C) + 30 min. (2~8°C) + 30 min. (RT) = 2 hr. total incubation time
- Catalog number:
- 27799
- configuration:
- 96 Determinations, 12x8 removable strips
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- design:
- Solid phase sandwich ELISA
- MSDS:
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Controls:
- None provided
- Sample types:
- Serum, EDTA plasma (Additional EIA buffer may be required depending on your sample type and is available for an additional charge. Please refer to the datasheet for the details)
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.14 - 9 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.07 ng/mL
- Species:
- Human
- Additional Info: