Enzyme immunoassay (ELISA) for the determination of IGFBP-1 in human serum, plasma (heparin, EDTA), amnion fluid, mother milk, urine, saliva, or cell culture media. For research use only, not for use in diagnostic procedures.<br><br>
The Insulin-like Growth Factors I and – II are free in body fluids and tissues but are bound to specific binding proteins. Until today seven different binding proteins (IGFBP-1 to –7) can be differentiated additionally several IGFBP-related proteins have also been detected. Bioavailability of IGF is regulated by these IGFBPs or better their proteolytic cleavage which reduces affinity to IGF. But the IGFBPs as well as their proteolytic fragments can also exert IGF-independent effects, like influencing cell migration or proliferation.<br><br>
IGFBP-1 (Placental Protein 12) consists of 234 aminoacids and has a molecular weight of approximately 25kDa. The coding DNA region is located on chromosome 7. IGFBP-1 is mainly synthesized by foetal and adult liver tissue and decidual endometrium. Intensity of Expression varies enduring menstruation with a maximal expression in the late secretory phase. IGFBP-1 is posttranslational modified by phosphorylation of serine residues 101, 119 and 169. Phosphorylation has physiological relevance as it increases affinity of IGFBP-1 to IGF. In adult humans phosphorylated IGFBP-1 of the liver is the predominant form in circulation. IGFBP-1 produced by endometrial tissue is significantly less phosphorylated than the liver originated form
- Assay Description:
- 1 hour incubation (RT) + 30 min. (RT) + 15 min. (RT) = 1 hour, 45 min. total incubation time
- Catalog number:
- E01
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, lyophilized
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum, plasma (heparin, EDTA), amnion fluid, mother milk, urine, saliva, or cell culture media
- Sample volume:
- 50 μL of properly diluted unknown / determination
- standards:
- 7 standards, lyophilized
- Standard range:
- 0 / 0.1 - 8.0 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.02 ng/mL
- Species:
- Human
