Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of IGFBP-1 concentrations in serum, body fluids, tissue homogenate or cell culture supernatants from mice. For research use only, not for use in diagnostic procedures.<br><br>
Insulin-like growth factor-binding protein 1 (IGFBP-1) also known as placental protein 12 (PP12) is a member of the Insulin-like growth factor-binding protein (IGFBP) family. It is synthesized in liver, secretory endometrium, and decidua. The IGFBP1 gene has 4 exons, spans 5.9 kb, and mapped to 7p13-p12. This protein has an IGFBP domain and a type-I thyroglobulin domain. It binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Leu and George (2007) concluded that IGFBP1 is a negative regulator of the p53/BAK-dependent pathway of apoptosis.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 30 min. (37°C) = 3 hours, 30 min. total incubation time
- Catalog number:
- BE69143
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immunesorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Insulin-like growth factor-binding protein 1 (IGFBP-1) also known as placental protein 12 (PP12)
- Protocol:
- Sample types:
- Serum, body fluids, tissue homogenate or cell culture supernatants from mice
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 31.2 - 2000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- <3 pg/mL
- Species:
- Mouse
