Skip to main content

Insulin-like Growth Factor-Binding Protein-2 (IGFBP-2)

$681.00
SKU:
E05
Bulk Pricing:
Buy in bulk and save
Adding to cart… The item has been added
Enzyme immunoassay (ELISA) for the determination of human IGFBP-2 in human serum, EDTA-plasma, cerebrospinal fluid, breast milk, amniotic fluid, saliva, and in cell culture medium. For research use only, not for use in diagnostic procedures.<br><br>
Insulin-like growth factors (IGFs) regulate the proliferation, differentiation, apoptose, cell adhesion and metabolism in various tissues and cell types. The IGF-I, which is produced mainly in liver under the influence of growth hormone (GH), regulates as hormone the linear growth of the bones and the process of sexual maturity, while IGF-II is mainly a growth factor of fetal tissue (11-13). The biological actions of IGF over the IGF-Type-I receptor are modulated variably through the IGF binding proteins (IGFBP-1 to-6) (14). IGFBP-2 is, after IGFBP-3, the second most frequent IGFBP in the human blood. IGFs, especially tumor typical pro-IGF-forms and hormones regulate the expression of IGFBP-2, GH effect is thereby inhibiting. At cellular level IGFBP-2 seems to stimulate the proliferation and dissemination of solid tumors via an IGF-independent mechanism (15,16).
Assay Description:
1 hour incubation (RT) + 30 min. (RT) + 15 min. (RT) = 1 hour, 45 min. total incubation time
Catalog number:
E05
configuration:
96 Determinations, 12x8 removable strips
controls:
2 controls, lyophilized
design:
Enzyme immunoassay (ELISA) technique
FDA Status:
For research use only, not for use in diagnostic procedures
MSDS:

Click for MSDS - ELISA
MSDS - Controls

notes:
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Protocol:

Protocol

Sample types:
Human serum, EDTA-plasma, cerebrospinal fluid, breast milk, amniontic fluid, saliva, and cell culture medium
Sample volume:
100 μL of properly diluted unknown / determination
standards:
5 standards, lyophilized
Standard range:
2 - 80 ng/mL
storage:
2 - 8 °C
sensitivity:
0.2 ng/mL
Species:
Human