Enzyme immunoassay (ELISA) for the determination of human IGFBP-2 in human serum, EDTA-plasma, cerebrospinal fluid, breast milk, amniotic fluid, saliva, and in cell culture medium. For research use only, not for use in diagnostic procedures.<br><br>
Insulin-like growth factors (IGFs) regulate the proliferation, differentiation, apoptose, cell adhesion and metabolism in various tissues and cell types. The IGF-I, which is produced mainly in liver under the influence of growth hormone (GH), regulates as hormone the linear growth of the bones and the process of sexual maturity, while IGF-II is mainly a growth factor of fetal tissue (11-13). The biological actions of IGF over the IGF-Type-I receptor are modulated variably through the IGF binding proteins (IGFBP-1 to-6) (14). IGFBP-2 is, after IGFBP-3, the second most frequent IGFBP in the human blood. IGFs, especially tumor typical pro-IGF-forms and hormones regulate the expression of IGFBP-2, GH effect is thereby inhibiting. At cellular level IGFBP-2 seems to stimulate the proliferation and dissemination of solid tumors via an IGF-independent mechanism (15,16).
- Assay Description:
- 1 hour incubation (RT) + 30 min. (RT) + 15 min. (RT) = 1 hour, 45 min. total incubation time
- Catalog number:
- E05
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, lyophilized
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum, EDTA-plasma, cerebrospinal fluid, breast milk, amniontic fluid, saliva, and cell culture medium
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 5 standards, lyophilized
- Standard range:
- 2 - 80 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.2 ng/mL
- Species:
- Human
