Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of Insulin in mouse serum, plasma, body fluids, tissue lysates or cell culture supernates. For research use only, not for use in diagnostic procedures.<br><br>
Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The human insulin gene contains 3 exons; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Insulin has a potent acute anti-inflammatory effect, including a reduction in intranuclear NF-kappa-B, an increase in IKB, and decreases in the generation of reactive oxygen species. It causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen inside these tissues, and improved insulin-stimulated glucose uptake after endurance training results from hemodynamic adaptations as well as increased cellular protein content of individual insulin signaling components and molecules involved in glucose transport and metabolism.
- Assay Description:
- 1 hour incubation (37°C) + 15 min. (37°C) = 1 hours, 15 min. total incubation time
- Catalog number:
- BE69091
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immune-sorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Mouse serum, plasma, body fluids, tissue lysates or cell culture supernates
- Sample volume:
- 50 μL properly diluted unknown / determination
- standards:
- 6 standards, lyophilized
- Standard range:
- 0 / 8 - 140 uIU/ml
- storage:
- 2 - 8°C
- sensitivity:
- < 5 uIU/ml
- Species:
- Mouse
