Sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of intact proinsulin in mouse and rat EDTA-plasma, serum and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Proinsulin is a precursor of insulin that is synthesized in the β cells of the islet of Langerhans of pancreas.<br><br>
Most of the proinsulin synthesized in the rough endoplasmic reticulum of β cells is stored in the secretory granules. Proinsulin is cleaved by prohormone converting enzyme (PC) 1/3 at the junction of the C-peptide with the B-chain of insulin, subsequently it is cleaved by PC2 at the junction of the C-peptide with the A-chain of insulin, consequently it is divided into insulin that consists of 21 amino-acid A chain and 30 amino-acid β chain and c-peptide that consists of 33 amino acid.<br><br>
It has been considered that the conversion rate from proinsulin to insulin in type 2 diabetes patients decreases due to dropping down of PC1/3 enzymatic activity by abnormal function of pancreatic β cells. Thus, intact proinsulin level in blood or the ratio of intact proinsulin and insulin (P/I ratio) can be considered to become a potential biomarker that reflects functional change of the PC1/3 activity at pancreaticβ cell.<br><br>
Especially, It has been known that the P/I ratio is a valuable pancreatic β cell evaluation method that does not require a loading test as it can be measured using blood samples in fasting.<br><br>
This ELISA kit can be used for measuring intact proinsulin, not including cleaved intermediate products, in mouse and rat samples.
- Assay Description:
- Overnight (2 - 8°C) + 1 hour (2 - 8°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
- Catalog number:
- 27706
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immunosorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the antibody for precise details.
- MSDS:
- Protocol:
- Sample types:
- Serum, EDTA-plasma, and cell culture supernatant
- Sample volume:
- 20 µL
- standards:
- 7 standards
- Standard range:
- 0 / 14.1 - 900 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 6.83 pg/mL
- Species:
- Mouse, Rat
- Additional info:
- References:
