Solid phase sandwich ELISA for the determination of mouse IL-6 (Interleukin-6) in serum, EDTA-plasma or cell culture media. For research use only, not for use in diagnostic procedures.<br><br>
Interleukin-6 (IL-6) is a cytokine that was discovered as a B-cell differentiation factor. IL-6 is not only an antibody-producing system, but its physiological activities, such as induction of acute-phase protein synthesis in hepatocytes, and growth stimulation based on its synergistic effect with IL-3, etc., have attracted great interest. Associations between IL-6 and pathology have been steadily demonstrated. For example, the growth factor in several diseases has been reported to be IL-6, and myeloma cells themselves have been reported to synthesize IL-6 and express IL-6 receptors. In addition, IL-6 has been suggested to contribute to a variety of inflammatory diseases and autoimmune diseases. This kit makes it possible to detect the IL-6 in mouse blood and in the supernatant of cultured cells with high sensitivity.
- Assay Description:
- Overnight incubation (4°C) + 30 min. (4°C) + 30 min. (RT) = Overnight + 1 hour total incubation time
- Catalog number:
- 27768
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA-plasma, cell culture supernatant
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 7.81 - 500 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 4.74 pg/mL
- Species:
- Mouse
- Products related:
- The control set is available for sale on request
- References:
