Enzyme immunoassay (ELISA) for the quantitative measurement of intact proinsulin in heparin or EDTA plasma. Measurements of proinsulin are used in the diagnosis and treatment of patients with type 2 diabetes. FDA exempt, can be used for in-vitro diagnostics.<br><br>
Proinsulin is a precursor molecule for insulin and is synthesized by the pancreatic β-cells. Under normal circumstances, virtually all proinsulin is cleaved at residues 32-33 and 65-66 to produce insulin during the formation of secretory granules. Some unmodified proinsulin is released into the circulation, though it is believed to have little or no biological activity. Increased concentrations of circulating proinsulin may occur in insulin-resistant syndromes such as type 2 diabetes and in patients with insulinoma. When used in conjunction with a highly specific insulin assay, it may provide useful information on changes in the processing of insulin in such situations.
- Assay Description:
- 2 hour incubation (RT) + 1 hour (RT) + 30 min. (RT) + 30 min. (RT) = 4 hours total incubation time
- Key Features:
- Calibrated against WHO International Standard (IRP 84/611)
Highly specific marker for insulin resistance in type 2 diabetes
High sensitivity detection system
Easy to use
High specificity for 'intact' proinsulin - Catalog number:
- IV2-102E
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, lyophilized
- design:
- Two-site enzyme-linked immunosorbent assay (ELISA) technique
- FDA Status:
- FDA exempt, can be used for in-vitro diagnostics
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Heparin or EDTA Plasma. Full recovery of intact proinsulin cannot be achieved from serum samples.
- Sample volume:
- 50 μL / determination
- standards:
- 5 standards, lyophilized
- Standard range:
- 0 / 1.2 - 110.0 pmol/L
- storage:
- 2 - 8 °C
- sensitivity:
- 0.3 pmol/L at 95% confidence
- Species:
- Human
