Solid phase sandwich ELISA for the determination of Mouse Leptin in serum, EDTA-plasma and supernatant of cell culture media. For research use only, not for use in diagnostic procedures.<br> <br>
The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman’s group at Rockefeller University originally identified the obesity gene that encodes for Leptin in 1994. Leptin is a 16 KDa secreted protein produced by the ob gene. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tends to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is also found that the protein acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. In addition, recent study shows Leptin acts as a potent inhibitor of bone formation through central nervous system.
- Assay Description:
- 60 min. incubation (37°C) + 30 min. (37°C) + 30 min. (RT) = 2 hr. total incubation time
- Catalog number:
- 27160
- Configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- None provided
- Design:
- Solid phase sandwich ELISA using two kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- MSDS:
- Sample Types:
- EDTA-plasma, Serum, and Cell culture supernatant
- Sample Volume:
- 100 μL of properly diluted unknown / determination
- Standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard Range:
- 0 / 12.5 - 800 pg/mL
- Sensitivity:
- 0.86 pg/mL
- Storage:
- 2 - 8 °C
- Species:
- Mouse
- References:
