Enzyme immunoassay (ELISA) for the determination of Leptin (Obese Protein) in mouse and rat serum and plasma (heparin, EDTA, citrate). For research use only, not for use in diagnostic procedures.<br><br>
Leptin, the product of the ob gene (1,2), is a recently discovered single-chain proteohormone with a molecular weight of 16 kD which is thought to play a key role in the regulation of body weight. Its amino acid sequence exhibits no major homologies with other proteins (1). Leptin is almost exclusively produced by differentiated adipocytes (3-5). It acts on the central nervous system, in particular the hypothalamus, thereby suppressing food intake and stimulating energy expenditure (2,6-9). Leptin receptors - alternatively spliced forms exist that differ in length - belong to the cytokine class I receptor family (10-12). They are found ubiquitously in the body (10,11,13,14) indicating a general role of leptin which is currently not fully understood. A circulating form of the leptin receptor exists which acts as one of several leptin binding proteins (15).<br><br>
Besides its metabolic effects, leptin was shown to have a strong influence on a number of endocrine axes. In male mice, it blunted the starvation-induced marked decline of LH, testosterone, thyroxine and the increase of ACTH and corticosterone. In female mice, leptin prevented the starvation-induced delay in ovulation (16). Ob/ob mice, which are leptin deficient due to an ob gene mutation, are infertile. This defect could be corrected by administration of leptin, but not through weight loss due to fasting (17), suggesting that leptin is pivotal for reproductive functions.<br><br>
All these actions may, at least in part, be explained by the suppressive effect of leptin on neuropeptide Y (NPY) expression and secretion by neurons in the arcuate nucleus (6,18,19). NPY is a strong stimulator of appetite (20,21) and is known to be involved in the regulation of various pituitary hormones, e.g. suppression of GH through stimulation of somatostatin (22,23), suppression of gonadotropins (23) or stimulation of the pituitary-adrenal axis (21).<br><br>
The most important variable that determines circulating leptin levels is body fat mass (24-26). Obviously, under conditions of regular eating cycles, leptin reflects the proportion of adipose tissue (27) showing an exponential relationship (37). This constitutive synthesis of leptin is modulated by a number of non-hormonal and hormonal variables. Stimulators in both rodents and humans are overfeeding (28,29), high fat diets (41), insulin (3,5,30-33) and glucocorticoids (5,34-36). Suppression has been shown for fasting (27), cAMP and beta--3- adrenoceptor agonists (35). From these findings it becomes clear that leptin is an integral component of various metabolic and endocrine feedback loops (38).
- Assay Description:
- 1 hour incubation (RT) + 1 hour (RT) + 30 min. (RT) + 30 min. (RT) = 3 hours total incubation time
- Catalog number:
- E06
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 control, lyophilized
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Mouse and Rat Leptin (Obese Protein)
- Protocol:
- Sample types:
- Serum and plasma (heparin, EDTA, citrate)
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 7 standards, lyophilized
- Standard range:
- 25 - 1600 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 10 pg/mL
- Species:
- Mouse, Rat