The IBL-America Lysozyme Assay Kit provides researchers with a sensitive assay to measure levels of lysozyme in human samples.
Lysozyme is one of the anti-microbial agents present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears and is also found in human milk. Lysozyme has 130 amino acids and its natural substrate is the bacterial cell wall peptidoglycan. Since synthesized by granulocytes and macrophages, lysozyme can act as a useful marker for myelomonocytic cells.
Increased levels of lysozyme in urine and serum are diagnostic indicators for acute monocytic leukemia and acute myelomonycytic leukemia. Elevated lysozyme levels were found in synovial fluids of the inflammatory arthritides and osteoarthritis. Salivary lysozyme, a marker for oral infection and hyperglycemia, might display a significant relationship with hypertension, an early stage of cardiovascular disease.
Increased serum lysozyme activity is also present in tuberculosis, sarcoidosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis and Crohns disease. Elevated lysozyme levels in urine and serum occur during severe renal insufficiency, renal transplant rejection, urinary tract infections, glomerulonephritis and nephrosis.
For research use only, not for use in diagnostic procedures.- Assay Description:
- 1 hour incubation (RT) + 15 min. (RT) = 1 hour, 15 min. total incubation time
- Catalog number:
- IB19131
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Solid phase direct sandwich ELISA technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Lysozyme (muramidase)
- Protocol:
- Sample types:
- Human plasma, serum, or stool
- Sample volume:
- 25 μL of properly diluted unknown / determination
- standards:
- 7 calibrators, ready to use
- Standard range:
- 0 / 1.0 - 40.0 ng/mL
- storage:
- 2 - 8 °C
- Species:
- Human
