Solid phase sandwich ELISA for the determination of Mac-2 binding protein (Mac-2bp) in mouse serum, EDTA plasma, tissue extract and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Mac-2 binding protein (Mac-2bp), known as 90K, is a highly N-glycosylated, secreted protein, identified as a ligand of Galectin-3. It is considered that through interaction with Galectin-3, Mac-2bp promotes homotypic cell-cell contact or regulates cell adhesion. And it has been reported that Mac-2bp levels in blood have associations with various cancers and chronic hepatic diseases such as NASH (Non-Alcoholic Steatohepatitis). This ELISA kit can measure concentration of mouse Mac-2bp.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (2 - 8°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27796
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich enzyme-linked immunosorbent assay (ELISA)
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Mac-2 binding protein (Mac-2bp)
- Protocol:
- Sample types:
- Mouse serum, EDTA plasma, tissue extract and cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0.78 - 50 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.09 ng/mL
- Species:
- Mouse
- Additional info:
- References:
