Solid phase sandwich ELISA for the determination of Rat MCP-1 in serum, urine and supernatant of cell culture media. For research use only, not for use in diagnostic procedures. <br><br>
MCP-1 (Monocyote Chemoattractant protein-1) is the basic protein consisting of 76 amino acids and is classified as C-C sub family of chemokines. MCP-1 shows specific chemotaxis to the monocyote and relates to the manifestation of desmosomal molecule on the surface of Monocyte, the Monocyte chemotaxis to the inflammation site, conjugation with endothelial cell and to the exudation to sub-endothelium. It is produced in chronic inflammatory diseases such as chronic arthritis and nephritis and in malignant tumors such as atherosclerosis and others and is thought to have important role to the regional exudation of Monocyte and macrophage.
- Assay Description:
- 1 hour incubation (37ºC) + 30 min. (37ºC) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 17176
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, urine, supernatant of cell culture media
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 50 - 3,200 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- Please see protocol for assay-specific performance characteristics
- Species:
- Rat
- References:
