Enzyme immunoassay (ELISA) for the determination of free Metanephrine and free Normetanephrine in plasma.<br><br>
Metanephrine (Metadrenaline) and Normetanephrine (Normetadenaline) are first extracted using an ion exchange matrix followed by an acylation process.<br><br>
The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450nm.<br><br>
Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 15 min. Centrifuge + 15 min. incubation (RT) + 15-20 hours (2-8°C) + 30 min. (RT) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
- Catalog number:
- IB89183R
- configuration:
- 96 Determinations, 12x8 removable strips (per analyte)
- controls:
- 2 controls, ready to use
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Please Inquire
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Plasma
- Sample volume:
- 500 μL
- standards:
- 6 calibrators, ready to use
- Standard range:
- Metanephrine: 0 / 36 - 3600 pg/mL; Normetanephrine: 0 / 48 - 4800 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- Metanephrine: 17 pg/mL; Normetanephrine: 23 pg/mL
- Species:
- Human
