Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of MICA in human tissue homogenate or cell culture supernates, serum and plasma should be validated by end users. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 30 min. (37°C) = 3 hours, 30 min. total incubation time.
- Catalog number:
- BE69184
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided.
- design:
- Sandwich enzyme-linked immunesorbent assay (ELISA) technique.
- FDA Status:
- For research use only, not for use in diagnostic procedures.
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- MHC class I polypeptide-related sequence A.
- Protocol:
- Sample types:
- Human tissue homogenate or cell culture supernates, serum and plasma.
- Sample volume:
- 100 μL of properly diluted unknown / determination.
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard.
- Standard range:
- 0 / 62.5 - 4000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- <10 pg/mL
- Species:
- Human
