Enzyme immunoassay for determination of MMP-3 concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only, not for use in diagnostic procedures.
Uniprot ID： P08254
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 10-20 min. (37°C) = 3 hours, 20 min. total incubation time
- Catalog number:
- IB08103
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immunesorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Other names:
- Matrix metalloproteinase-3 (MMP-3), Stromelysin-1
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Human serum, plasma, cell culture supernatant and other biological samples
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.156 - 10 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.094 ng/mL
- Species:
- Human
