Solid phase sandwich ELISA for the determination of mouse Total Angiotensinogen ELISA in serum, EDTA-plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures. <br><br>
Angiotensinogen is a precursor protein of Angiotensin and it is cleaved by the renin enzyme secreted from juxtaglomerular cells (JG Cells) which are located at the afferent arterioles of kidneys. Once cleaved, Angiotensin I is produced. After that it is cleaved by Angiotensin-Converting Enzyme (ACE) and Angiotensin II is produced. <br><br>
In the Renin-Angiotensin-Aldosterone System (RAAS), Angiotensin II has an important role of arterial contraction of the entire body, and stimulates adrenal cortex to secret Aldosterone. Aldosterone deposits Na in the body and because of the feature, the volume of blood circulation, cardiac output and peripheral vascular resistance (PVR) are increased. Renin is inhibited after blood pressure is increased. <br><br>
In the past, plasma renin activity (PRA), active renin concentration (ARC) and its combination were used for assessment of renin activity in blood marker in RAAS, however, the impact of posture such as erect position or seated position, and also variability in a day were not properly considered in the assessment. According to study lead by Kobori et al., it has been suggested that the ratio of des AI Angiotensinogen / Intact Angiotensinogen can be calculated through the measurement of both Intact Angiotensinogen and total angiotensinogen in blood and it can be much stabile renin activity biomarker.<br><br>
Measurement of Angiotensinogen is useful in research of primary aldosteronism, novel RAAS inhibitor and cardiovascular and renovascular disease.<br><br>
More recently, the association between COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and RAAS including ACE inhibitors and Angiotensin receptor inhibitors (ARBs) has been actively researched around the world. <br><br>
This kit can quantitatively measure Total Angiotensinogen (both Intact Angiotensinogen and Des AI Angiotensinogen cleaved by renin) in mouse serum, plasma and Cell culture supernatant samples.
- Assay Description:
- Overnight (2 - 8°C) + 60 min. (2 - 8°C) + 30 min. (RT) = Overnight and 1 hour, 30 min. total incubation time
- Catalog number:
- 27103
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA-plasma, or cell culture media. ( €» Additional EIA buffer may be required depending on your sample type and is available for an additional charge. Please refer to the datasheet for the details.)
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.16 - 10 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.03 ng/mL
- Species:
- Mouse
- Additional info:
