Sandwich enzyme-linked immunesorbent assay (ELISA) for the determination of CD40 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernates. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 90 min. incubation (37°C) + 1 hour (37°C) + 30 min. (37°C) + 25 min. (37°C) = 3 hours, 25 min. total incubation time.
- Catalog number:
- BE69009
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided.
- design:
- Sandwich enzyme-linked immunesorbent assay (ELISA) technique.
- FDA Status:
- For research use only, not for use in diagnostic procedures.
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Mouse serum, plasma, body fluids, tissue lysates or cell culture supernates.
- Sample volume:
- 100 μL of properly diluted unknown / determination.
- standards:
- 8 calibrators, serially diluted from 1 prepared lyophilized calibrator.
- Standard range:
- 0 / 15.6 - 1000 pg/mL
- storage:
- 2 - 8°C
- sensitivity:
- <1pg/ml
- Species:
- Mouse
