Enzyme immunoassay for determination of Mouse IL-2 concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only, not for use in diagnostic procedures.
Uniprot ID： P04351
Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation. IL-2 also promotes peripheral development of regulatory T cells (Tregs). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 10-20 min. (37°C) = 3 hours, 20 min. total incubation time
- Catalog number:
- IB08131
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immunosorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Interleukin-2 (IL2), T-cell growth factor
- Sample types:
- Serum, plasma, cell culture supernatant, and other biological samples
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 15.625 - 1000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 9.375 pg/mL
- Species:
- Mouse
