Sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of total insulin in mouse and rat EDTA-plasma and serum. For research use only, not for use in diagnostic procedures. <br> <br>
Insulin is peptide hormone that is released from the beta cells of the islets of Langerhans of pancreas. It is synthesized as 86 amino-acid precursor “proinsulin” and it is cleaved by C-peptide and insulin that consists of 21 amino-acid A-chain and 30 amino-acid β chain that is linked by disulfide binding due to activation of prohormone conversion enzyme (PC2, PC3) in secretory granule. Insulin deposits glucose and promotes synthesize protein in skeletal muscles. It also has physiological function such as suppress gluconeogenesis in liver and it has been known as an only hormone that can decrease blood glucose. Insulin is constantly released to maintaining the level of blood glucose while it is also released in large amount stimulated by incretin and glucose for suppressing rapidly increased blood glucose after meals. However, lack of insulin secretion and decreasing insulin sensitivity are observed and eventually high blood glucose is observed in diabetic conditions.<br><br>
This ELISA kit can measure mouse and rat total insulin including proinsulin and low concentration of insulin in fasting using tiny amount of samples.
- Assay Description:
- 60 min. incubation (RT) + Overnight (2 ~ 8°C) + 60 min. (2 ~ 8°C) + 30 min. (RT) = Overnight +2 hr. 30 min. total incubation time
- Catalog number:
- 27705
- Configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- None provided
- Design:
- Sandwich ELISA
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- MSDS:
- Sample Types:
- EDTA-plasma and Serum
- Sample Volume:
- 100 μL of properly diluted unknown / determination
- Standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard Range:
- 0 / 1.25 - 80 pg/mL
- Sensitivity:
- 0.50 pg/mL
- Storage:
- 2 - 8 °C
- Species:
- Mouse, Rat
- Additional info:
