Sandwich ELISA with a combination of two antibodies against Titin N-Fragments. A titin fragment containing N-terminal region in mouse serum can be measured. For research use only, not for use in diagnostic procedures.<br><br>
Titin (connectin) is a protein that consists of 34,350 amino-acid and specifically expresses in a cross-striated muscle. The molecular weight of human titin is 3,816 kDa and it has been known that as the largest protein among the existing proteins in a living body. It is one of sarcomere structured protein that is a minimum unit of myofibrillary protein and it has a role as an elastic protein for recovering the length of shortened sarcomere by its contraction. It has been known that titin is cleaved by protein metabolic enzymes such as calpain and matrix metalloprotease if muscles are damaged.<br><br>
Titin has been researched in the field of sports medicine, cardiac disease, NAFLD, sarcopenia and frailty and it is also considered that it can be a candidate as a non-invasive biomarker for monitoring status of muscles.<br><br>
- Assay Description:
- Overnight incubation (2-8C) + 60 min. (2-8C) + 30 min. (RT) = Overnight + 1.5 hours total incubation time
- Catalog number:
- 27604
- Configuration:
- 96 Determinations, 12x8 removable strips
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Design:
- Solid phase sandwich ELISA
- MSDS:
- Please inquire
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Sample types:
- Mouse Serum
- Sample volume:
- 100 uL of properly diluted unknown / determination
- Standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard Range:
- 0 / 75 - 4800 pmol/L
- Storage:
- 2 - 8 °C
- Sensitivity:
- 21.20 pmol/L
- Species:
- Mouse
