Solid phase sandwich ELISA for the determination Human N-ERC/Mesothelin in EDTA plasma. For research use only, not for use in diagnostic procedures.<br><br>
Erc has been identified as a gene showing stronger expression in cancer-affected renal tissue than in normal renal tissue in Eker rats (a rat model of renal cancer). The human homologue of the protein encoded by this gene is called MPF (megakaryocyte potentiating factor) or mesothelin. This protein is detected especially prominently in mesothelial cells, and its involvement has been suggested in the development of mesothelioma, making it a promising tumor marker. In humans, involvement of this protein has also been suggested in the development of pancreatic, ovarian and pulmonary cancers, etc. The protein is expressed as a GPI anchor-type membranous protein (about 71 kDa in molecular weight), which is digested by a furin-like protease to yield fragments about 31 kDa and 40 kDa in size. We have established a system for the assay of this protein, referring to the 31 kDa fragment as N-ERC/Mesothelin. This ELISA kit can measure N-ERC/Mesothelin in human plasma samples.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27190
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- EDTA plasma
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.07 - 4.4 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.017 ng/mL
- Species:
- Human
- References:
