Solid phase sandwich ELISA for the determination of rat N-ERC / Mesothelin in serum, EDTA plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Erc has been identified as a gene showing stronger expression in cancer-affected renal cell than in normal renal tissue in Eker rats (a rat model of renal cancer). The human homologue of the protein encoded by this gene is called MPF (megakaryocyte potentiating factor) or mesothelin. This protein is detected especially prominently in mesothelial cells, and its involvement has been suggested in the development of mesothelioma, making it a promising tumor marker. In humans, involvement of this protein has also been suggested in the development of pancreatic, ovarian and pulmonary cancers, etc. The protein is expressed as a GPI anchor-type membranous protein (about 71 kDa in molecular weight), which is thought to be digested by a furin-like protease to yield fragments about 31 kDa and 40 kDa in size. We have established a system for the assay of this protein, referring to the 31 kDa fragment as N-ERC/Mesothelin and 40 kDa fragment as C-ERC/Mesothelin. The kit is a complete kit for the quantitative determination of rat N-ERC/Mesothelin.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (4°C) + 30 min. (RT) = 3 hours total incubation time
- Catalog number:
- 27765
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA plasma and cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.08 - 5 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.01 ng/mL
- Species:
- Rat
- Products related:
- The control set is available for sale on request.
- References:
