Solid phase sandwich ELISA for the determination of human Napsin A in serum, urine and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Napsin A (NAPSA) is a pepsin-like aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. Napsin A is expressed as a single chain protein with the molecular weight of approximately 38 kDa. Immunohistochemical studies revealed high expression levels of Napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs. This ELISA kit can measure human Napsin A.
- Assay Description:
- Overnight incubation (4°C) + 30 min. (4°C) + 30 min. (RT) = Overnight + 1 hour total incubation time
- Catalog number:
- 27758
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, urine and cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 3.91 - 250 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 2.92 ng/mL
- Species:
- Human
- References:
