Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of NGF concentrations in serum, body fluids, tissue homogenate or cell culture supernates from rat. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 30 min. (37°C) = 3 hours, 30 min. total incubation time.
- Catalog number:
- BE69198
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided.
- design:
- Sandwich enzyme-linked immunesorbent assay (ELISA) technique.
- FDA Status:
- For research use only, not for use in diagnostic procedures.
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Nerve growth factor (NGF).
- Protocol:
- Sample types:
- Serum, body fluids, tissue homogenate or cell culture supernates from rat.
- Sample volume:
- 100 μL of properly diluted unknown / determination.
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard.
- Standard range:
- 0 / 15.6 - 1000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- <1 pg/mL
- Species:
- Rat
