Solid phase enzyme immunoassay (ELISA) with human native nucleosomes isolated from the eukaryotic cell line HeLa for the determination of antibodies against nucleosomes and their components dsDNA and histones in human serum. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time
- Catalog number:
- AE29003
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 positive control and 1 negative control, ready to use
- design:
- Solid phase enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum
- Sample volume:
- 100 µL of properly diluted (1:101) unknown / determination
- standards:
- 6 calibrators, ready to use. Alternatively, a cut-off calibrator, ready to use, may be used for qualitative determination.
- Standard range:
- 0 / 3 - 300 U/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 1.0 U/mL
- Species:
- Human
