Solid phase sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of Periostin in human serum, EDTA plasma, heparin plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Periostin is approx. 90kDa, an extracellular matrix protein that expresses on an outer membrane of bone and a periodontal membrane. It has a function as a ligand ofαVβ3 andαVβ5 and it involves cell migrant and cell adhesion. In addition, it has been known that it binds extracellular matrix protein such as collagen, fibronectin and tenascin C. In recent year, it has been reported that periostin is elevated in pancreatic cancer. Some research and development of drugs and in vitro diagnostic maker using neutralizing antibody have been conducted as it has also been suggested that periostin may involves with allergy such as heart failure and bronchitis.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27262
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich enzyme-linked immunosorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum, EDTA plasma, heparin plasma and cell culture supernatant
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0.31 - 20.00 ng/mL
- storage:
- 2 - 8°C
- sensitivity:
- 0.15 ng/mL
- Species:
- Human
- Additional info:
