Solid phase enzyme immunometric assay (ELISA) for the determination of PMN elastase in EDTA or citrated plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 1 hour incubation (RT) + 1 hour (RT) + 20 min. (RT) = 2 hours, 20 min. total incubation time
- Catalog number:
- IB79187
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, lyophilized
- design:
- Solid phase enzyme immunometric assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Plasma (EDTA or citrated), exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma
- Sample volume:
- 100 μL of properly diluted (1:100) unknown / determination
- standards:
- 8 calibrators, serially diluted from 1 prepared lyophilized calibrator
- Standard range:
- 0 / 15.6 - 1000 ng/mL
- storage:
- 2 - 8°C
- sensitivity:
- 0.2 ng/mL
- Species:
- Human