Prorenin Receptor Protein & The RAA System
Posted by Brandon Savela on Aug 16th 2018
(Pro)renin receptor/(P)RR is a common receptor protein between renin and prorenin. When prorenin binds to (P)RR, it achieves a binding ability to angiotensinogen and gains a catalytic activity for conversion from angiotensinogen to ansiotensin I in a comparable level with renin. Additionally, when the (P)RR is stimulated by binding to prorenin, its intracellular signaling progresses. Through this, (P)RR research is believed to have an important implication in building new strategies for the suppression of an overactivated RAA (Renin-Angiotensin-Aldosterone) system.
(P)RR is a 39 kDa, single transmembrane receptor protein and it is reported that the 29 kDa soluble form is generated by cleavage of (P)RR with furin. Thus the assay of soluble (P)RR in blood or urine samples is expected to provide new knowledge to help clarification of disease mechanisms and development of new tools for diseases. This ELISA kit can measure the concentration of human soluble (pro)renin receptor in blood or urine samples.
Soluble (Pro)renin Receptor ELISA
Catalog Number | 27782 |
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Species | Human |
Design | Solid phase sandwich ELISA using 2 kinds of highly specific antibodies. |
Standards | 8 standards, serially diluted from 1 prepared lyophilized standard. |
Controls | None provided. |
Sample Types |
Human: serum, EDTA-plasma, urine. Mouse and Rat: serum, EDTA-plasma. Cell culture supernatant. |
Sample Volume | 100 μL of properly diluted unknown / determination. |
Assay Desc. | Overnight incubation (4°C) + 1 hour (4°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time. |
Standard Range | 0 / 125 - 8000 pg/mL |
Sensitivity | 24 pg/mL |
Storage | 2 - 8 °C |
Configuration | 96 Determinations, 12x8 removable strips |
FDA Status | For research use only, not for use in diagnostic procedures. |