Competitive enzyme immunoassay (ELISA) for the determination of progesterone in rat and mouse serum or plasma. For research use only, not for use in diagnostic procedures.<br><br>
Progesterone (4-pregnene-3, 20-dione) is a C21 steroid hormone containing a keto-group (at C-3) and a double bond between C-4 and C-5. Like other steroids, it is synthesized from cholesterol via a series of enzyme-mediated steps. Progesterone is a female sex hormone of primary importance in ovulation, fertility and menopause. It is particularly important in preparing the endometrium for the implantation of the blastocyte and in maintaining pregnancy. The rate of progesterone secretion may be affected by the degree of pro-gestational activity of the uterus and the level of circulating LH. Analyses suggest that progesterone acts as an anti-glucocorticoid in rat adipose tissue in vivo, attenuating the glucocorticoid effect on adipose tissue metabolism. Furthermore it could be demonstrated that progesterone alone may be a valuable agent for management of postmenopausal osteoporosis.<br><br>
In female rodents, the determination of progesterone is a useful marker in evaluating and monitoring the state of the reproductive functions and pregnancy as well.
- Assay Description:
- 1 hour incubation (RT) + 30 min. (RT) = 1 hour, 30 min. total incubation time
- Catalog Number:
- IB79183
- Configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- None provided
- Design:
- Solid phase enzyme-linked immunoassay (ELISA)
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Please inquire
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample Types:
- Serum and plasma
- Sample Volume:
- 10 µL
- Standards:
- 6 standards, ready to use
- Standard Range:
- 0 / 0.4 - 100 ng/ml
- Storage:
- 2 - 8 °C
- Sensitivity:
- 0.156 ng/ml
- Species:
- Mouse,Rat
