Sandwich enzyme immunoassay (ELISA) for the determination of prolactin (PRL) in human serum. FDA exempt, can be used for in-vitro diagnostics.<br><br>
Prolactin (PRL) is a polypeptide hormone synthesized by the lactotropic cells of the anterior pituitary gland. Structurally, it is similar to two other polypeptide hormones namely, growth hormone and placental lactogen. PRL is a polypeptide containing 199 amino acids, while growth hormone and placental lactogen each have 191 amino acids. There is approximately 100 µg of prolactin in the human pituitary gland, which is a very small amount when compared to growth hormone, which is present at 8–10 mg.<br><br>
The target organ of prolactin is the breast (mammary gland). Its main physiological action is not only to initiate but also to sustain lactation. The hypothalamus secretes dopamine, which has a direct effect of inhibition of the secretion of PRL. If dopamine is not available or absent the secretion of PRL is autonomous.
- Assay Description:
- 1 hour incubation (RT) + 15 min. (RT) = 1 hour, 15 min. total incubation time
- Catalog number:
- IB59110
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Sandwich enzyme immunoassay (ELISA) technique
- FDA Status:
- 510(k) exempt - IVD
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum
- Sample volume:
- 25 µL / determination
- standards:
- 6 calibrators, ready to use
- Standard range:
- 0 / 0.77 - 123 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- Please see protocol for assay-specific performance characteristics
- Species:
- Human
