Enzyme immunoassay for determination of Rat Ghrelin concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only. Not for use in diagnostic procedures.
Uniprot ID： Q9QYH7
Ghrelin, also known as GHRL, is a neuropeptide hormone belonging to the motilin family. It is the ligand for the growth hormone secretagogue receptor type 1 (GHS-R), expressed by cells in the hypothalamic ventromedial nucleus and arcuate nucleus . Ghrelin is synthesized as a preprohormone by ghrelinergic cells in the gastrointestinal tract; proteolytic cleavage yields a 28-amino acid peptide hormone, which undergoes obligate n-octanoylation at serine 3 by the enzyme ghrelin O-acetyltransferase (GOAT) . Binding of n-octanoyl ghrelin to GHS-R stimulates growth hormone release, while simultaneously exerting multiple neuroendocrine affects that influence appetite, gastric motility and energy balance.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 10-20 min. (37°C) = 3 hour, 15 min. total incubation time
- Catalog number:
- IB08063
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Serum, plasma, cell culture supernatant, and other biological samples
- Sample volume:
- 100 μL of properly diluted sample
- standards:
- 8 standards, from 1 vial of lyophilized stock
- Standard range:
- 0 / 7.812 - 500 pg/mL
- sensitivity:
- 4.688 pg/mL
- storage:
- 2-8 °C
- Species:
- Rat
