RGMa (Repulsive Guidance Molecule a) is a GIP-anchored protein, about 33 kDa, and it is implicated in axonal guidance and neural tube closure of embryonic retina. It has reported as a potent inhibitor of axonal regeneration in the adult central nervous system (CNS). It is speculated that RGMa inhibits neurite outgrowth by activating the RhoA-Rho kinase signaling pathway in vitro. While, it has reported that, in a rat SCI (spinal cord injury) model, neurite outgrowth is enhanced by administration of anti-RGMa antibody to lesion site, resulting they are improved in motor functional recovery. Thus function analysis of RGMa is attracting attention in a research field of axonal regeneration of CNS. This antibody detects rat RGMa and can be used for western blotting, immuno-precipitation and FACS analysis. And it is also reported that administration of the antibody to cerebellar granule cells of 6 - 8 days-old rat, in vitro, neutralizes RGMa-dependent inhibition of neurite outgrowth. This product cannot be used for neutralization test. For research use only, not for use in diagnostic procedures.
- application:
- WB, IP, FCM
- Catalog number:
- 28045
- Datasheet:
- formulation:
- Lyophilized product from 1% BSA in PBS containing 0.05% NaN3
- immunogen:
- Synthetic peptide of a part of Rat RGMa (DFQAFRANAESPRR)
- MSDS:
- notes:
- For research use only, not for use in diagnostic procedures.
The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the antibody for precise details. - Protocol:
- purification:
- Purified with antigen peptide
- size:
- 100 µg
- specificity:
- Reacts with rat RGMa (confirmed by western blotting with transfectant)
- storage:
- Lyophilized product, 5 years at 2 - 8 °C; Solution, 2 years at -20 °C.
- Species:
- Rat
- Host:
- Rabbit
- References:
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