Solid phase sandwich ELISA for the determination Mouse/Rat sAPPα in serum, EDTA plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures. <br><br>
Alzheimer’s disease (AD) was first reported by A. Alzheimer, a German neuropathologist in 1907 and is considered as a major factor of dementia. It is known that Amyloid β (Aβ; which is major constituent of senile plaque) is cleaved from Amyloid Precursor Protein (APP; which exists in three main isoforms, APP695, APP751, and APP770) by β-secretase and subsequent γ-secretase. The production of soluble APPβ (sAPPβ) by β-secretase cleavage corresponds to Aβ production accordingly, so it is desired to measure sAPPβ in parallel with Aβ. On the other hand, it is considered that in the metabolic pathway of APP, APP is first cleaved by α-secretase rather than β-secretase normally to produce soluble APPα (sAPPα) and subsequently P3 is cleaved from the remaining C-terminal fragment by γ-secretase. In recent research, there are several attempts to apply the inhibitor of β-secretase and the activation of α-secretase for AD treatment. As stated above, it is useful for research of AD to measure sAPPα as well as sAPPβ. This kit can measure Mouse/Rat sAPPα. Note: Please pay attention in sample selection since this kit also measures full-length APP.
- Assay Description:
- Overnight incubation (4°C) + 1 hour (4°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time.
- Catalog number:
- 27419
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided.
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies.
- FDA Status:
- For research use only, not for use in diagnostic procedures.
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Soluble Amyloid Precursor Protein Alpha.
- Protocol:
- Sample types:
- Serum, EDTA plasma and cell culture supernatant.
- Sample volume:
- 100 μL of properly diluted unknown / determination.
- standards:
- 8 calibrators, serially diluted from 1 concentrated calibrator.
- Standard range:
- 0 / 4.1 - 260 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.6 pg/mL
- Species:
- Mouse
- Additional info:
- References:
