Solid phase sandwich ELISA for the determination of sAPPβ-w in mouse plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Alzheimer's disease (AD) was first reported by A. Alzheimer, a German neuropathologist in 1907 and is considered as a major factor of dementia. Regarding the cause of Alzheimer’s disease, the Amyloid cascade hypothesis that suggests accumulating of peptide so-called Amyloidβ (Aβ) in the brain become a trigger and it causes death of neuron is widely supported. It is known that Aβ is produced by cleaving by 2 types of secretase called β secretase and γ secretase. Firstly it is cleaved by β secretase and subsequently cleaved by γ secretase from Amyloid Precursor Protein “APP” that is membrane protein consists of 695, 751 or 770 amino acids.Since soluble APPβ (sAPPβ) produced by β-secretase cleavage corresponds to Aβ production accordingly, it is desired to measure sAPPβ in parallel with Aβ. On the one hand, it is considered that the metabolic pathway of APP is normally started by cleaving by α-secretase rather than β-secretase and soluble APPα (sAPPα) is produced and subsequently molecule called “P3” is cleaved by γ-secretase.Current drug research and development of Alzheimer’s disease has been progressing based on the Amyloid cascade hypothesis and especially Aβ production suppressed by regulation of secretase activity is focused. Measuring sAPPα and sAPPβ in parallel with Aβ for research and evaluation of secretase inhibitor is valuable.This kit can only measure soluble sAPPβ in these 2 types of soluble APP in mouse samples.
- Assay Description:
- Overnight incubation (2-8°C) + 1 hour (2-8°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time.
- Catalog number:
- 27416
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided.
- design:
- Solid phase sandwich enzyme-linked immunosorbent assay (ELISA) technique.
- FDA Status:
- For research use only, not for use in diagnostic procedures.
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Soluble Amyloid Precursor Protein (APP) - beta (wild type).
- Protocol:
- Sample types:
- Mouse plasma, cell culture supernatant.
- Sample volume:
- 100 µL of properly prepared unknown / determination.
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard.
- Standard range:
- 13 - 800 pg/mL
- storage:
- 2 - 8°C
- sensitivity:
- 2.87 pg/mL
- Species:
- Mouse
- Additional info:
