Solid phase enzyme immunoassay (ELISA) for the separate qualitative determination of IgG antibodies against eight different cellular and nuclear antigens in human serum. The wells are separately coated with recombinant human 100 kDa PM-Scl, 70 kDa U1-snRNP, SS-B, SS-A 52 kDa, SS-A 60 kDa, Scl 70, centromere protein B (CenpB), Jo-1 and highly purified native human Sm. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time
- Catalog number:
- AE29002
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 negative control, ready to use
- design:
- Solid phase enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum
- Sample volume:
- 100 µL of properly diluted (1:101) unknown / determination
- standards:
- 1 cut-off calibrator, ready to use
- Standard range:
- Cut-off calibrator is used to calculate cut-off value for the interpretation of results of unknowns
- storage:
- 2 - 8 °C
- sensitivity:
- 1.0 U/mL
- Species:
- Human
