Solid phase sandwich ELISA for the determination of Human SCF in cell culture medium. For research use only, not for use in diagnostic procedures. <br><br>
Stem cell factor (SCF) and its receptor KIT play an important role in various biologic phases, such as hematopoiesis, reproduction, and regeneration. Studies in white spotting and steel mice have shown that functional SCF and c-Kit are critical in the survival and development of stem cells involved in hematopoiesis, pigmentation and reproduction. Interaction of SCF with c-Kit rapidly induces receptor dimerization and increases in autophosphorylation activity.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (37°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27141
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Cell culture medium
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 50 - 3200 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 7.14 pg/mL
- Species:
- Human
- Products related:
- The control set is available for sale on request
- References:
